Western blot analysis H1N1/HA1 recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human H1N1/HA1 antibody (1:500). (1:1000), (1:5000). Proteins were visualized using a goat mouse secondary antibody conjugated to HRP and an ECL detection system.
ELISA H1N1/HA Antibody (1ug/ml) specifically recognizes H1N1/HA1 recombinant protein, but not interacted H3N2/HA1 and H5N2/HA1 recombinant protein in ELISA.
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